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RXSOL
Stains Indicator Reagents

AURAMINE

SKU: RXSOL-69-6906-001

Auramine is an aniline dye used as a disinfectant and an antiseptic agent. It is weakly fluorescing and binds specifically to certain proteins.  It is very soluble in water and soluble in ethanol.

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Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Dosage

Auramine O is Basic yellow 2 is a diarylmethane dye used as a fluorescent stain. In its pure form, Auramine O appears as yellow needle crystals.
Auramine O can be used to stain acid-fast bacteria (e.g. Mycobacterium), where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain. It can also be used as a fluorescent version of Schiff reagent.

Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis. It can be also used as an antiseptic agent.

Technical Specifications

Specifications:

 

MOLECULAR FORMULA    C17H21N3·HCl 
MOLECULAR WEIGHT   303.83
COLOUR CRUDE YELLOW POWDER
DYE CONTENT 85 %
ABSORPTION MAXIMUM λ (ph 6.0) 431-433 nm
ABSORPTIVITY (A 1%/1cm λ MAX ph 6.0 ON DRIED MATERIAL  850-1000
SUITABILITY FOR MICROSCOPY PASSES TEST
LOSS ON DRYING AT 1100 C MAX 2 %

About

RXSOL — Uganda Chemical is the East African supply arm of RX Marine International (RXSOL), supplying industrial chemicals and solvents since 1996. We deliver bulk solvents, degreasers, acids and alkalis, glycols, water-treatment chemicals, and laboratory reagents to customers across Uganda and the wider East African region.

Contact info

Industrial chemicals & solvents supplier since 1996.

mail@ugandachemical.com
+91 80699 76670

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