Lab Chemical
TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE
SKU: RXSOL-60-6605-763
Tricine is a commonly used electrophoresis buffer and is also used for the resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement.
Application
Tricine gels are suitable in isolating hydrophobic proteins from 2D gel for mass spectrophotometric analysis. It is also helpful to isolate membrane protein complexes from biological membranes. When urea is added in stacking gel it can easily separate two different proteins of the same molecular weight
Dosage
Glycine is used as a bulking agent buffers. Glycine at low concentrations prevents pH decrease in solutions. It also stabilizes a protein when present in an amorphous
