CAPSO BUFFER FOR BIOCHEMISTRY
CAPS buffer is a zwitterionic buffer widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. The high pH of this buffer makes it useful for the transfer of proteins with
Application
A simple, sensitive, and robust method has been developed for the separation and quantification of glutamate in beverages and foodstuffs. Samples were derivatized with 5 × 10−4 M fluorescein isothiocyanate. Glutamate was separated by capillary electrophoresis and detected by laser-induced fluorescence with 488-nm radiation from an argon ion laser.