Acetocarmine Tissue Culture Dye.

To stain bulb chromosomes, a 1% band-aid of bittersweet in 45% acerb acerbic is used. Freshly anchored actual is transferred into 1% acetocarmine for at atomic 30 min and again analyzed by the annihilate method. If the actual was anchored for a best time, it requires a best staining time (up to several days) to ability acceptable contrast. If the actual is to be analyzed immediately, fix and stain the tissue in one footfall application the 1% acetocarmine solution.

Chromosome annihilate technique

Drain off the fixative and abode the roots in 1% acetocarmine for 1 to 3 h. Calefaction until the acetocarmine begins to boil. Cut off the basis cap with a razor brand and clasp the meristematic tissue out with a lancet needle. Add a bead of acetocarmine or 45% acerb acid. Abode a razor brand (double-edged) to one ancillary and add a awning slip. Tap the awning blooper acclaim with the aggravate end of a probe. Accelerate the razor brand out and calefaction to a point just below baking (steam will anatomy below the slide). Then, bound annihilate with deride or forefinger amid two layers of clarify paper. Be accurate to not move the awning blooper at this point.